Polymorphic regions between strain A721 and UBC21 were identified from the P. blakesleeanus genome website (version 1.1; http://genome.jgi-psf.org/Phybl1/Phybl1.home.html), with those that could be detected by changes in restriction enzyme sites chosen to develop PCR-RFLP markers.
Insertions, duplications, inversions, and deletions can cause changes in restriction fragments.
In soybean, reproducible non-random changes in restriction length polymorphic markers induced by in vitro culturing of root explants have also been documented.
Restriction factors and accessory proteins are engaged in an evolutionary "molecular arms race" consisting of multiple rounds of host adaptation, virus counteraction, and host re-adaptation, resulting in accumulation of amino acid changes in restriction factor-accessory protein interaction interfaces (Daugherty and Malik, 2012).
The pulsotypes A1 and A2 were isolated more than a year after pulsotype A and genetic events in a strain leading to changes in restriction site are not surprising, according to Tenover et al. [ 47] and indicated one circulating strain in the region.
A parks department spokesman, Randall Sawyer, said last week that no change in restrictions on boats was planned.
The observed change in restriction pattern is the result of a mutation of the BamHI restriction site at ≈0.93 map units of the genome.
Therefore, if a reanalysis is conducted with a change in restriction enzyme pair to obtain other SNP makers, the sequence adapters need to be redesigned for individual samples.
Martin Sosland, who represents the team, said, "Certainly there were changes in the restrictions on the debtors".
