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(H) Western blot showing changes in protein levels of EMT markers in miR-564 transfected MDA-MB-231 cells.
This highlights the fact that transcriptional regulation is not always reflected by similar changes in protein levels, and that observation of a protein does not necessarily mean that the activity it is implicated in is taking place.
Assessing changes in protein levels and function is the most efficient way to evaluate the mechanisms underlying sensitivity and resistance to targeted agents.
We used quantitative RT-PCR to assess the mRNA levels and designed a semiquantitative Western blot assay to analyze changes in protein levels between the three stages studied.
As a group of ligand-induced proteins, different subtypes of mammalian Notch (Notch1-4) are sensitive to subtle changes in protein levels.
The increase in phospholamban or Na+/Ca2+ exchanger mRNAs did not, however, result in changes in protein levels.
Two-dimensional gel electrophoresis (2-DE) is a most commonly used proteomics technology for monitoring global changes in protein levels in plants (Agrawal and Rakwal 2006).
This approach identified 120 significant changes in protein levels.
As expected, changes in protein levels lagged slightly behind the observed changes in transcript levels.
For the remaining candidate genes, only slight changes in protein levels were observed in hepatocyte supernatants.
Alterations in gene expression do not always parallel changes in protein levels or cellular function.
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