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Introducing the mutations W320A and Y328F caused changes in pathway bias, with different patterns of change between ligands.
Further study of the pathways through which NET4/Tmem53 affects cell cycle regulation would not be practical using exogenous expression because transfection efficiencies were too low (5 10%) to be able to quantify changes in pathway components by Western blot.
Metabolomic assay of pool levels are better for detecting the presence of pathways and detecting the site of pathway blockages, but changes in pathway fluxes may not result in increases or decreases of pool levels.
Interestingly, changes in pathway activity did not affect cell proliferation.
(PDF 86 kb) Additional file 10: Dose-dependent changes in pathway activity.
Studies of inter-modular relationships may reveal changes in pathway dependencies due biological perturbations.
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This study was designed to assess smooth muscle function and motility in defunctionalized colonic segments and subsequent changes in pathways responsible for gastrointestinal motility.
Specifically, we identified gene expression changes in pathways which are essential for Ca2+-mediated exocytosis.
Alternatively, expression differences may represent compensatory changes in pathways and networks.
GenMAPP 2.1 was used to visualize the age-associated expression changes in pathways found significant by GSEA.
GenMAPP 2.1 (www.genmapp.org) was used to visualize the age-associated expression changes in pathways found significant by GSEA.
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