Moreover, changes in mRNA-specific translation rates can occur, and such changes can alter protein production rates independently of transcriptional activity.
Knowing that the OSCs induced compound-specific changes in mRNA abundance profiles, we sought to determine if these changes could be related to specific functional pathways using gene ontology (GO) enrichment analysis.
Among the 401 genes identified, further work is necessary to determine how altered GmSKRPs levels cause such massive but specific changes in mRNA processing.
This indicates that probes targeting PolyA+ transcripts are not appropriate for detecting specific changes in mRNA metabolism, as they represent a general population of transcripts.
To identify specific changes in mRNA abundance associated with differential TCDD-induced toxicities observed in male and female C57BL/6 mice, the hepatic transcriptome was profiled.
Independent follow-up validation identified one hit able to induce modest, yet specific, changes in mRNA expression indicative of a partial differentiation to stumpy forms in monomorphs.
Cell cultures are prepared to initiate conjugation by starvation in very dilute buffer (typically 10 mM Tris), a treatment that also induces morphological alteration to a "fast swimmer" cell shape [ 55], changes in chromatin structure [ 56] and histone modification [ 57], and general and specific changes in mRNA abundance [ 14, 58].
Thus significantly more putative miRNA targets, 56 and 388 respectively, were identified by miRNA-specific enrichment Ago2 IPs than from the miRNA-specific changes in mRNA levels.
doi: 10.1186/s13059-018-1551-9. Johnson EL, Robinson DG, Coller HA. (2017) Widespread changes in mRNA stability contribute to quiescence-specific gene expression patterns in a fibroblast model of quiescence.
First, it was important to examine whether HU can cause widespread non-specific changes in mRNA splicing patterns.
The elucidation of the complete genome of T. brucei strain 927 in 2005 [ 11] affords the opportunity to examine global changes in mRNA expression using a gene-specific approach.
