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Duration of antibiotic treatment was calculated in days with administration of an antibiotic regardless of class or changes in antibiotics.
All patient information was collected from medical charts, including the types and doses of the antibiotics administered, all the bacteria isolated, changes in antibiotics after culture results, and the duration of antimicrobial use during hospitalization.
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However, an increase in frequency over the past years has been suggested due to changes in antibiotic usage.
Six percent of the participants (25/391) required changes in antibiotic therapy, most commonly for persistent fever (6/25, 25%).
Clinical response to therapy and culture results are usually used to direct changes in antibiotic therapy during the inpatient phase of management.
Contrasting changes in antibiotic activity to changes on the other properties we have measured will thus allow us to test the hypothesis that selection has played specifically on Flhdc dependent traits.
With regard to the antibiotic resistance phenotype, we were able to confirm small changes in antibiotic resistance when sdiA is expressed from a plasmid, but saw no differences in antibiotic resistance between a wild-type strain and an sdiA mutant of E. coli K-12, EHEC, or S. Typhimurium, at 30°C or 37°C, on solid agar or motility agar.
Thus, we have confirmed the previously published results that plasmid-encoded sdiA can lead to small changes in antibiotic resistance but we observe no effect of AHL or sdiA on antibiotic resistance when sdiA is expressed from its natural position in the chromosome.
Changes in antibiotic resistance phenotypes of prevalent strains were predicted by trends in antibiotic consumption.
However, changes in antibiotic policies are probably mandatory to reduce the occurrence of multi-resistant bacteria.
Patterns of incidence and changes in antibiotic susceptibilities were tabulated and statistical analysis was performed.
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