However, supernumerary chromosome are the only cytotypic changes reported to occur in section Pachyneurium, suggesting that the genome size differences between different accessions observed here might be related to the presence of extra-chromosomal DNA, or that somatic changes in the accessions sampled may have been extensive.
We also surveyed the accessions for changes in the open reading frame of FRI, which is located in a region syntenic to chromosome 5 of A. thaliana (Irwin et al. 2012).
A population analysis including an examination of its ecological characteristics as well as comparing patterns of synonymous and non-synonymous changes in coding sequences of all accessions were also used to detect the natural selection of genes.
These include changes in centromere protein F (GenBank accession no. NM_016343), replication factor C (GenBank accession no. BC000149), and p16INK4A (GenBank accession no. AF115544).
Analyses of transcriptome changes in ozone-sensitive and resistant accession will provide important clues for understanding the molecular changes governing the plant responses to ozone.
Also these genetic markers can be applied periodically to monitor changes in heterogeneity and heterozygosity in the accessions, as they are routinely reproduced for maintenance.
In this work we gained a deeper insight into the molecular basis of legume resistance to parasitic plants by analyzing proteome changes in roots of two M. truncatula accessions in response to O. crenata infection.
We have accomplished a combination of two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry analysis to compare the protein changes in roots of two M. truncatula accessions displaying different resistant phenotypes, in the hope of contributing to a better understanding of the molecular basis of this plant-parasitic plant interaction and those underlying resistance in legumes.
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Position of mutation Base substitution Change in amino acid AF332093a, AF369029a, AF440570a, AF4114664 Nil Nil Nil Table 6 Changes in vp281 gene sequence in GenBank Accession nos.
