Sentence examples for changes cells were from inspiring English sources
Although both triterpenes displayed similar changes, cells were more sensitive to erythrodiol.
For the analysis of morphological changes, cells were treated with H35N or pre-treated with zVADfmk for 2 hr followed by H35N treatment and stained with PI (2 µg/ml) for 5 min. Live cells were analyzed by FACS.
To test whether SFKs are involved in MCF-7 phenotypic changes, cells were treated with the SFK-specific inhibitor PP2.
To quantify the morphological changes, cells were stained and examined using automated fluorescence microscopy and image analysis software [ 18, 27].
To exclude ex vivo neutrophil density changes, cells were separated within 2 hours of collection using Ficoll-Hypaque™ Plus (GE Healthcare Biosciences, Uppsala, Sweden) density gradient.
To evaluate possible morphological changes cells were treated with 1 μM of PDA-66 for 48 h and analyzed by light microscopy.
In response to extracellular changes, cells are well known to utilize the ubiquitin/proteasome system to control levels of transcription factors, cell-cycle proteins or membrane receptors.
The following day, medium was changed, cells were treated as indicated and harvested.
The medium was then changed, cells were maintained for 24 h, and then medium was collected to quantify ANGPTL2 protein by ELISA.
At 48 h after the medium change, cells were selected in medium containing 400 μg ml−1 of G418 (GIBCO BRL) and G-418-resistant clones were established.
