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The motivation behind SPIED was in the first place to define expression changes as relative to the average across the experimental series and thereby facilitate automatic curation and secondly to map expression changes to a uniquely defined set of genes thus allowing for the database to combine data from multiple platforms.
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All fold change values were shown as relative to the younger stage and control (CTR) condition that was indicated as 100% of gene expression.
Due to relatively large variability in baseline values, the results are usually expressed as changes relative to baseline [ 28], which was also the case in this study.
Mutations, deletions and insertions (defined as changes relative to the reference sequence) are recorded for each submitted sequence.
The projected temperature changes are always presented as simulated changes relative to the simulated temperatures of the individual models for the baseline period 1961 1990.
Changes in gene expression were reported as percentage changes relative to controls.
In order to normalise the results, data were calculated as change relative to day 1.
PD values are presented and analyzed as change relative to pre-dose values.
Images were captured using a Fuji-film LAS-300, densitometry performed using AlphaEaseFC4.0 software, and data expressed as change relative to control.
In all cases, luciferase activity was corrected for protein content and expressed as change relative to the control group stated in the figure legend.
Results were reported as fold changes relative to proximal late control and graphed as mean ± SE.
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