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These changes were validated by quantitative RT-PCR (Fig. 4D).
Relevant expression changes were validated by qPCR experiments.
The ability of the FE models to simulate the moisture diffusion and suction variations in relation to climate changes is validated through two case studies involving field observations.
Much larger gene changes were validated in a number of novel genes as exemplified by TMEM176A, which showed 35-fold enrichment in the cortex and 30-fold enrichment in hippocampus of FRL animals relative to FSL.
Proteome changes were validated by western blot analysis, which confirmed that microtubule-associated protein, RP/EB family, member 1 (Mapre1; GeneID: 13589), eukaryotic translation elongation factor 2 (EF-2; GeneID: 13629) (Figure 5A) and heat shock protein 70 kDa (Hsp70; GeneID: 15511) (Figure 5B) increase following LT challenge.
These changes were validated by orthogonal immunoblotting.
Gene expression changes were validated by RT-PCR.
Arsenic-mediated expression changes were validated by QPCR.
The effect of reading on VIQ changes was validated by structural brain changes.
Second, gene expression changes were validated in a second, independent set of animals (Set 2).
Can these changes be validated by other experimental approaches like RT-PCR?
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