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All we meant was to convey the common-sense assumption that in molecular evolution "big changes" are selected against more frequently and more stringently than "small changes".
It may well be that certain epigenetic changes are selected for under some environments, but that will have been selected for previously.
Taken together with the study from Stewart et al. these results provide evidence for selection of some mtDNA mutations in the mouse female germ line and suggest that these severe mtDNA mutations encoding non-synonymous changes are selected against at the organelle level.
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While natural selection was accepted as the method by which changes were selected, the mechanism by which the changes initially occurred was unclear.
The response to setpoint changes is selected by means of the feedforward compensator or via a prefilter.
The time points of most significant changes were selected for more detailed analysis of cisplatin response in the breast cancer cell line MCF-7.
Genes with largest standardized fold changes were selected as informative.
Only genes with more than two-fold changes were selected.
Metabolites showing at least twofold changes were selected for further analysis.
Only clear and conserved quantitative changes (>2-fold changes) were selected, drastically reducing the number of final candidate spots.
Fifteen variants causing amino acid changes were selected based on their co-segregation pattern within the family (Table 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com