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The expression analysis showed that 65 miRNAs, comprising 42 mature miRNAs and 23 miRNA stars, exhibited statistically significant changes (a threshold of correct P-value < 0.001 and fold-change > 2.0) in at least one of the three life-stages during E. granulosus development [Additional file 1: Table S9].
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In order to get a value for the significant change, a threshold can be applied to the MAD/MAFs.
As a second strategy, nonlinear changes in a threshold over time can be observed by using a threshold tracking paradigm.
If the atmospheric delay has faster spatial changes, a lower threshold will be selected.
The strategy ranking changed at a threshold value of P = 0.0025 corresponding to a RR of prostate cancer death of 0.76.
If a (k) changes less than a threshold, such as a very small δ, with respect to a (k−1), we consider the iteration converges.
Adding a further stringency by analyzing the fold changes, where accepting a threshold >1 for the absolute log2-ratio reduced the list to 105 significant genes.
Why were twofold changes used as a threshold for analyses performed on transcripts or gene elements?
As a consequence, the properties of the solution were observed to change to reach a threshold value.
Differentially expressed transcripts were identified by fold-change screening with a threshold of ≥2-fold.
The expression-fold change was set as 1.5 as a threshold for changes of potential biological importance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com