To quantify RGS17 expression after genotype changes, we performed RT-qPCR in selected subclones with different genotypes.
To provide biological insight into the observed metformin-induced methylation changes, we performed Ingenuity Pathway Analysis (IPA; see Albitar et al.25).25
To unveil these WM changes, we performed high resolution diffusion tensor imaging (DTI) and correlated the results with histological analysis of adjacent slices taken from the spinal cords of presymptomatic mice.
Conversely, to measure the unique effect of phonological working memory improvements on reading speed changes - controlling for training-induced attentional changes - we performed a three-step, fixed entry, multiple regression analysis in which the predictors order were: (1) age (2), attentional and (3) phonological working memory changes.
To measure the unique effect of attentional improvements on reading speed improvements - controlling for training-induced phonological working memory changes - we performed a three-step, fixed entry, multiple regression analysis in which the predictors order were: (1) age (2), phonological working memory and (3) attention changes.
To estimate mutation-induced structural changes, we performed structure remodeling for LATS1/2 based on two AGC family proteins, ROCK and PKC.
To correct the 222 marker candidates for age and duration of DM related proteomic changes, we performed a non-parametric analysis of the variances (Kruskall-Wallis test).
To further support that the MSChUCB showed differential marker expression in the differentiated vs. undifferentiated conditions which can be correlated with the observed morphological changes, we performed immunohistochemistry.
Since the replacement of hydrophilic glutamine with hydrophobic alanine may cause relatively large changes, we performed the Q453E mutation to eliminate the effect of the side chain rotation of Gln453.
In order to prove whether the higher accumulation of intact RbcL in the wild type is due to higher protein stability caused by conformational changes, we performed a protease assay based on similar experiments with RbcL from Chlamydomonas.
