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Following overnight incubation the medium was changed to regular growth medium.
MEFS were infected at 80 bacteria per cell. 1 h post infection gentamycin (50 µg/ml) was added for 1 h to kill extracellular bacteria before the medium was changed to regular media containing 25 µg/ml gentamicin and primosin.
After 6 h the culture medium was changed to regular medium containing antibiotics and serum.
After 6 h, the medium was changed to regular culture medium.
For etoposide treatment, cells were incubated in medium containing 2 µM etoposide (Sigma Aldrich) for 1 hour, washed and changed to regular medium and incubated for 72 hours before analysis.
In case of Pseudomonas ESI, systemic antibiotic treatment with oral ciprofloxacin monotherapy or in combination with intraperitoneal (IP) gentamicin or ceftazidime was given for at least 14 days and the prophylactic topical mupirocin was changed to regular topical gentamicin 1% cream.
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With the approval of the special committee, a provisional or non-degree student whose status changes to regular degree may transfer up to two registration units completed while in provisional or non-degree status.
A Pass/D/Fail course may be changed to a regular course or a regular course to a Pass/D/Fail course until mid-November in the fall term and until mid-March in the spring term regardless of whether a student has received a midterm grade for the class before the relevant date.
As previously reported, the smeared pattern for cnt1 and imr1 regions in wild-type was lost in the mis12 kinetochore mutant and changed to a regular pattern (Fig. 7E), consistent with the disruption of chromatin structure at core regions [48].
Free feeding, which is having food always available, can be an easy way to feed, but be aware that some dogs will overeat and then need to be changed to a regular amount and schedule.
Regular attendance may be related to distal factors e.g., childhood abandonment and neglect or to more proximal events e.g. life events or period of depression precipitating a change to regular attendance in primary and secondary care settings.
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