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Non-adherent cells were removed after 1 week and medium (with 10% FBS) was changed subsequently every four days.
After 48 h, medium was removed and replaced with 10% FBS DMEM and changed subsequently every 48 h.
In the next section the findings of the two themes identified for the current paper, specifically 'coping (strategies)' and 'impact of vision loss on daily life', are combined to describe how vision loss affected the person from the time directly after becoming visually impaired and how this changed subsequently.
This suggests that the DNA-repair function, which is a key feature of Cry5, was the ancient function of this Cry and that the function of Cry4 changed subsequently after the genome duplication and has no functional representative in present invertebrates.
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Since this kind of collinearity is likely to change with every single individual or breed added to the training population, the prediction equation will change subsequently.
Although cerebral palsy may not remain a stable diagnosis before five years of age [ 49], a diagnosis of severe cerebral palsy (defined below) at two years of age is unlikely to change subsequently [ 50].
They say relatively low numbers of assisted suicides immediately after the law was changed have subsequently risen.
It was changed and subsequently broadcast, but in a period of high tension between ministers and the BBC, Rees-Mogg was very much on the establishment side.
The conformation change subsequently triggers HCR to generate plentiful bonding sequences of magnetic nanoprobes.
Understanding the scale of geologic time may provide a context in which to structure key course concepts, such as major evolution radiations, plate tectonics, and climate changes; subsequently, students are better able to understand underlying geologic processes and systems.
However, since the method iteratively improves the halftone frame, there is no explicit control on how the halftone frame changes subsequently, and there is a possibility for a scenario like this.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com