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However, natural variation in Nod factor receptors that causes changed specificity has rarely been documented.
However, natural variation in NFRs that causes changed specificity has rarely been documented.
Mora et al [ 13] changed specificity between RF1 and RF2 by swapping 16 residues in the mRNA recognition domain using such a strategy.
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DcAvd provides a scaffold that can be modified not only to alternate affinity for a ligand, but also to change specificity – in other words to favour another ligand molecule instead of biotin.
To change specificity, an amino acid replacement must affect affinity for RE genotypes differently.
Moreover, the results of Grundy et al. [ 9, 12] imply that in principle the T-Box can change specificity easily.
In this study TaqII bifunctional REase was shown to change specificity in the presence of a cofactor analogue, SIN (enhanced by DMSO).
In the Drosophilid lineage, the majority of inhibitory serpin orthologues show a striking conservation of the critical the proteinaseteinase cleavage sites, indicating that these inhibitors do not readily change specificity for their target proteinases.
Heterologous hapten desmycosin (DMN) based conjugate being immobilised on the plates changed the specificity to group recognition (cross-reactivity for TMN was 103.4%).
Hybridization is an alternative to directed evolution that has successfully changed the specificity of several small serine recombinases.
In addition, it is still an open question if factors have changed host specificity of the hybrid.
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