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You somehow forget that you have even changed medium.
He also changed medium depending on his health.
The vehicle group changed medium at the same time without drug treatment.
Culture medium was replaced 72 hr later and changed medium twice weekly thereafter.
We changed medium every two days until selection was complete and distinct colonies formed (2-3 mm diameter).
To investigate this scattering phenomenon further, we seeded cells at low density and changed medium when the cells were approximately 25% confluent.
Similar(53)
After 12 h the medium was changed to medium containing pD3, 7DHP and pregnenolone.
Prior to infection, culture medium was changed to medium without serum and antibiotics.
After 2 days in vitro, medium was changed to medium prepared as above but without serum.
After 24 h of transfection, the medium was changed to medium with chrysotile fibers.
24 h afterward, medium was changed to medium without supplements.
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