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Maximum SPS activity (measured wih saturating substrates, Vmax) only changed in two lines, whilst activity of the enzyme measured in the assay that contained limiting substrate concentration (Vmax/Km) and as a consequence 1/Km increased in all the lines.
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No significant bias in the number of up or down regulated genes was observed among the 79 and 52 genes that reproducibly changed in three lines at the S0∶1 and S5∶6 generations, respectively.
Among them, the expression of three proteins changed in all six cell lines, eight proteins changed in five, while 27 proteins changed in four of six different transgenic ES cell lines.
Add in two lines for the tail.
Besides, we examined CT45A1-mediated cell morphological and functional changes in other two lines, which do not express CT45A1, one is breast cancer cell line Hs578T and the other is gastric cancer cell line AGS.
Mcl-1 levels were not changed in the four cell lines.
Bid levels were not changed in the four cell lines treated with MLS-2438.
We analyzed copy-number changes in five MA lines (MA7, MA16, MA19, MA50 and MA66), 25 adaptive recovery populations (7A-E, 16A-E, 19A-E, 50A-E, 66A-E), and five additional control populations (C1-C5) that were propagated for the same period as the adaptive recovery populations but had not undergone a prior MA phase.
CCND1 transcript levels were increased only in LS174T cells, whereas HATH1 transcript levels did not changed in any of three cell lines.
Always in one line.
A single-gene resolution aCGH profiling was integrated with microarray-based gene expression profiling data to distinguish genetic copy number alterations that were strongly associated with transcriptional changes in two neuroblastoma cell lines.
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