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Two of the most changed genes were ones involved in the detection of pheromones, the hormonelike scents with which fruit flies signal their activities.
They used GO category and MeSH term to analyze the microarray dataset and discovered that those changed genes were related with cell death, apoptosis, oxidative stress, inflammation, and neurotransmitters in the brain.
Significantly changed genes were overrepresented in a number of pathways, most notably the cytoskeleton functional category.
We report that a significant proportion of changed genes were found near the targeted gene.
For ercc1 and neb mutants, the changed genes were distributed more widely on chromosome 7 and 2 respectively.
Microarray results for significantly changed genes were confirmed by quantitative reverse transcriptase PCR (qRT-PCR) (Figure 1B).
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The changed genes are listed in Tables S1 and S2.
Of all fatty acids studied, the number of significantly changed genes was highest for C22 6, followed by C18 3.
Next, we determined in which biological processes these dose-dependently changed genes are involved based on their Gene Ontology (GO Biological Processes).
The number of changed genes was plotted for the length of the relevant chromosome and the position of the targeted gene indicated.
The ordered list of significantly changed genes was subjected to GO term analysis using DAVID functional annotation tool (Table S1) [34], [35], [35].
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