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Of all fatty acids studied, the number of significantly changed genes was highest for C22 6, followed by C18 3.
The number of changed genes was plotted for the length of the relevant chromosome and the position of the targeted gene indicated.
The ordered list of significantly changed genes was subjected to GO term analysis using DAVID functional annotation tool (Table S1) [34], [35], [35].
The highest number of statistically significantly changed genes was found after treatment with C22 6 (519, P<0.01), followed by, in turn, C18 3 (400), C18 2 (287), C20 5 (280) and C18 1 (114) (Figure 2 and table S1).
Using a cutoff of 1.5-fold changes in RNA expression, WPMY-Vec control cells had only 8 genes that were differentially expressed in the presence of DHT and the graph showing the range of these changed genes was generated by the GeneSpring program and is shown in Figure 2A.
The magnitude distribution of the significantly changed genes was illustrated by MA plot analysis.
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Two of the most changed genes were ones involved in the detection of pheromones, the hormonelike scents with which fruit flies signal their activities.
They used GO category and MeSH term to analyze the microarray dataset and discovered that those changed genes were related with cell death, apoptosis, oxidative stress, inflammation, and neurotransmitters in the brain.
The changed genes are listed in Tables S1 and S2.
We report that a significant proportion of changed genes were found near the targeted gene.
Significantly changed genes were overrepresented in a number of pathways, most notably the cytoskeleton functional category.
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