Conversely, many GO groups of genes with changed expression in other tissues were not found to have changed expression in the lung.
On the contrary, MACROCALYX was expressed in all 4 whorls, and had a significantly changed expression in both the splayed out anthers and the anomalous ovaries, respectively.
The microarray results revealed changed expression in most functional gene categories for all three strains.
Among all genes, 1,524 unique Affymetrix targets changed expression in both Panc-1 and A549 after treatment with TGFβ.
Nevertheless, in each tissue there was a large number of genes that had significantly changed expression in response to diet (Figure 2B).
Per time-point, the number of up- and down-reglated genes was largely balanced, and genes changed expression in the same direction in both HF conditions (Table S1).
With the use of a 2DE-approach (BN/SDS-PAGE), protein maps revealed that in quadriceps, out of 300 Coomassie-blue detected and matched spots, 19 proteins exhibited changed expression in AQP4−/− compared to WT mice.
Among the non-CQ treated parasites, when compared with the parental line 106/1K76, only 45 genes significantly changed expression in one or both mutant parasites that passed the stringent threshold (Table 1).
These results are consistent with those from a recent microarray analysis that found none of the canonical courtship genes changed expression in male flies after brief exposure to females.
Comparisons between lists of contigs with significantly changed expression in the different treatments and the generation of preliminary Venn diagrams was performed using the FiRe macro in Excel® [59].
Heat map representation of the hierarchical clustering of 320 genes with at least 2-fold changed expression in SH-SY5Y cells treated with SUN N8075 at 3 µM for 3, 7 and 13 h out of the top 10% of fluorescence intensity in the non-treated control is shown in Fig. 2B and listed in Table S2.
