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After 24 hr, medium was changed as specified and subsequently refreshed every other day.
NuMat was prepared according to published protocol [ 14] with modifications as specified below.
DNA was isolated according to Austerberry and Yao (8), with modifications as specified below.
§provides a prompt, complete conference report as specified below.
Measure distances as specified below and multiply them by two.
This approximation defines error bars of the deduced values as specified below.
The samples were treated for isolation and identification of V. cholerae as specified below.
At the end of the experiments, all the specimens were processed for histological observations, as specified below.
Experiments were conducted in phosphate buffer, pH 7, at concentrations between 10 and 50 mM as specified below, in the absence of any additional salt.
For correlation with various neuronal phenotypes we performed immunocytochemistry on a variety of Gal4 lines crossed with UAS-GFP, as specified below.
These data sets were analyzed as specified below.
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