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After 6 h incubation with the constructs, the medium was changed as above with the optimization medium supplemented with sodium pyruvate, l-glutamine, and d-glucose to a final volume of 500 μL.
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The MBC or MFC was regarded as the lowest concentration of test sample which did not produce a colour/turbidity change as above, indicating no microbial growth.
The basis of the harmony had changed, as noted above, from the perfect intervals (unison, fourth, fifth, octave) to the triad, or chord such as C E G, built of thirds above a root, or bass note.
The colors were changed as mentioned above as well.
The effective threshold range also changes as the above conditions evolve.
These were full-factorial for five network sizes, two recombination rates, and five rates of environmental change, as above.
We performed a SIFT analysis on the amino acid changes as above.
The MBC or MFC was regarded as the lowest concentration of test sample which did not produce a colour or turbidity change as above.
After 2 days in vitro, medium was changed to medium prepared as above but without serum.
Using the same change of variable as above, we arrive at the following second-order differential equation with functional boundary conditions: (4.11).
Samples were washed in 3 changes of cold buffer as above and the sample was then incubated at 60°C in a 2% agarose solution in 0.1 M sodium cacodylate buffer pH 6.8.
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