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Cell culture medium was changed and cell samples were analyzed on a daily basis.
Six hours after transfection, OPM was changed and cell lysates for western blotting or Trizol extracts for total RNA isolation were made 72 hr later.
The shape of the cell changed and cell arrangement was extensively disrupted, as can be seen by comparing Figures 6a, 7a and 6b, 7b.
After 24 h of treatment, fresh complete culture medium was changed and cell colonies were allowed to grow for 10 days.
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After cell seeding (12 to 24 h), cell culture media were changed, and cells were cultivated in cell culture media with material eluates.
The medium was changed and cells were split 24 h later.
The next day, the medium was changed and cells were infected as described below.
Thereafter, cell culture medium was changed and cells were treated with 0, 5, 10, 15 and 20 µmol/L SFN.
Twenty four hours prior to stimulation, the medium was changed and cells were cultured in DMEM supplemented with 0.5% FBS.
Media was changed and cells were treated with fresh media, in the presence or absence of GFs.
The medium was changed and cells were cultured for additional 48 h before cell adhesion assay.
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