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Overexpression of UFM1 did not change the localization of UFM1, still cytoplasmic and ER (Figure 4F).
As a result, the WT FBXO7 N-terminal 40 amino acid peptide, but not the T22M-mutant, was able to change the localization of profilin from cytoplasm to nucleus (Figure 6).
Indeed, KLK did not change the localization of CD43-YFP when the cells were pretreated by neuraminidase (Fig. 6).
Next, their binding properties were employed to change the localization of the respective fluorescent proteins within cells.
Furthermore, expression of mutant SOD1 does not change the localization of SIRT1, which is mainly nuclear, and of SIRT2, which is mainly cytosolic, as in nontransgenic mice.
However, these genes are expressed in a variety of cell types in different tissues, and thus complete knockout of these transporters would globally change the localization of their substrates, and thus their specific function at the BBB may be obscured.
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Rab39B co-localizes with Htt and changes the localization of Htt, protecting it from degradation.
The Nucleo-cytoplasmic Transport, which requires three factors, eIF Set, Dhh1p, and Pat1p, changes the Localization of the mRNA from nucleus to P body.
Finally, similar to Mps1 [21], we show that during mitosis hypoxia changes the localization of Polo, suggesting a role for this protein in mediating the hypoxic response.
The two other transport processes are the inverse of each other: P Body-Ribosome Transport changes the Localization of the mRNA from P body to ribosome and induces translation initiation, while Ribosome-P body Transport does the opposite, repressing translation.
This mutation changed the localization of FMRP from cytoplasmic to nuclear.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com