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Table 3 Colour fastness properties Dyes Chitosan concentration Wash fastness Wet crocking fastness Light fastness Colour change Staining on cotton Staining on nylon Acid Red 138 0 3–4 4 3 4 3 4 4 0.11 4 3–4 3–4 3 4 0.05 4 4 3–4 3–4 4 0.3 4 4 4 3–4 4 0.5 4 4 4 4 4 0.5 4 4 4 4 4. Pretreated cotton/nylon samples were tested for fabric properties such as air permeability and, tensile strength.
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Colonized monolayers demonstrated a time-dependent series of changes: staining with labelled phalloidin identified accumulation of actin at the cell junctions; ZO-1 staining revealed a loss of Caco-2 tight junction integrity; and Hoechst staining showed condensation and fragmentation of nuclear material consistent with apoptosis.
Treatment with PDGF changed staining pattern from diffuse cytosolic to membrane localized, indicating activation of RhoA.
In addition to the change in distribution of LCA+ cells, there is also a change in staining intensity and morphology.
The minimum inhibitory concentration (MIC) was defined as the lowest extract concentration that can inhibit bacterial growth, as indicated by resazurin staining (dead bacterial cells are not able to change the staining color by visual observation blue to red).
Co-staining with CD63, a tetraspanin that is abundant in late endosomes and lysosomes, revealed an interesting change in staining pattern in cells expressing mutant CHMP2B.
Rapamycin was also used to enhance autophagy and resulted in a similar change in staining pattern as noted for serum starvation (Figure 10B).
At this early time-point of recovery, we did not observe any change in staining for the oligodendrocyte marker Rip within the subcortical white matter or SVZ (Data not shown [26]).
This change in staining pattern indicates a profound pathology at this stage.
We quantified this change in staining intensity by measuring the mean gray value of the images.
The data relating to the change in staining following chemotherapy appear statistically significant for VEGF expression (p < 0,05), but not for CD31 (p > 0,05).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com