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To observe the temporal change, samples were collected after heating for 30, 60, and 300 s.

The structure of the phase-change samples was checked by X-ray diffraction [8].

When a culture attained steady state (after 5 volume changes), samples were collected for estimation of dry cell mass, fluoride release and residual 4-FCA.

For platelet identification and glycoprotein IIb/IIIa (GPIIb/IIIa) expression changes samples were stained with 6 μl anti-GPIIb/IIIa PE (phycoerythin -conjugated monoclonal antibody (CD41-phycoerythin -conjugatedulter, Krefeld, Germonoclonal

The fold change between samples was calculated between the crossover points of each linear trend line at the ΔCt threshold.

We defined a miRNA as differentially expressed if the fold change between samples was higher than 4 and P < 10−4 (Bonferroni corrected).

Color change in exposed samples was calculated with respect to the average measured L*a*b* of the unexposed swatches.

Relative quantification (fold change) between different samples was then determined according to the 2−ΔΔCt method (Livak and Schmittgen 2001).

After the last resin change, the samples were transferred into gelatin capsules with fresh LR White.

Genes with less than 2.5 fold change across samples were eliminated from consideration.

MMP-12 was stable in sputum with only 2 samples showing >25% change, both samples being 24 hour room temperature samples.