The quality of data varies due to changes in reagents, equipment, testing utilities, calibration methods, operators and/or analysts.
Samples that have been assigned to a role as starting material can be changed into reagents during the planning of the reaction.
The effect of a change of reagent lot on the measured values of a commercial immunoturbidimetric HbA1c assay (A1C3) was investigated.
Cell medium was changed and reagents were added fresh every other day for seven days.
Process drift or process improvement, introduced through automation of procedures or changes in reagents, equipment, operator, or platforms, may involve relatively subtle effects on microarray data.
In all, block-like structures are seen in every brain region, and almost always correspond to experimental batches, suggesting that they arose from changes in reagents, hybridization protocols, chip types, or scanning conditions.
To study the impact of mithramycin on the production of MMP-13 by chondrocytes in their native cartilage matrix, human and bovine cartilage explants were maintained in low-serum (0.01%) medium and exposed to mithramycin (150 nM) and IL-1β for 15 days with changes of reagents every 2 days.
For other SN hits that did not decrease pairing of the 359 bp repeat, it is possible that changes in reagents, including S2 cell population and dsRNA sequences, or effects that are specific to euchromatin and/or the MSL complex could account for the lack of correlation between the two analyses.
These relate in part to defining a cut-off value to differentiate between positive and negative results and to the sensitivity of the test to changes in reagents and laboratory conditions, which is particularly relevant in longitudinal studies and repeat examinations.
There have been no major changes in reagent or instrumentation in the flow cytometry crossmatch (FC-XM) since its description in 1983.
