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While Fe-SP at concentrations between 300 nM and 1 µM proved to be highly cytotoxic to SKOV-3 and OVCAR-3 cells, treatment of HeLa cells or primary fibroblasts with 1 µM Fe-SP did not result in any change of viability.
RECIST cannot accurately reflect the change of viability of a tumour cell.
The data (in fluorescence units from the microplate reader) for the test wells were normalized to the assay control (DMEM only), and bystander effects were calculated as a change of viability in the irradiated group compared to the unirradiated group per sample.
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The time-dependent changes of viability in control and LHON cybrids, preincubated with 12 mg/ml of 2,5-HD reported in figures 1C and 1D, showed a similar behaviour.
The time-dependent changes of viability in control and LHON fibroblasts treated with 12 mg/ml of 2,5-HD (figure 3C and 3D) reveals that, consistently with the other experiments, the 11778/ND4 and 14484/ND6 mutations associated with haplogroup J1 were the most sensitive.
In this condition, the change of seed viability, physiochemical properties including protein content, fat acidity, and some antioxidant enzyme activities were observed at 10 day intervals during treatment.
Whereas NAC (0.5 mM to 20 mM) did not result in a significant change of cell viability and VEGF level).
There was no significant change of cell viability between pre and post 300 μg/ml PMS treatment for 36 h.
In addition, OBs stimulated by MSU reduce their proliferation rate without change of their viability, and MSU crystals remain intact inside OBs.
CHO cells were treated with 300 μg/ml PMS for 36 h, and there was no significant change of cell viability between pre and post PMS treatment (Additional file 3: Figure S2d), which indicates that PMS has no side effect on normal cells.
Additionally, in our study, although IGF-1 treatment alone could promote cell viability, there were no significant changes of cell viability during AMP-induced cell death in both breast cancer cells (data not shown).
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