Sentence examples for change of buffer from inspiring English sources

Concentrates were run on a Superdex 200 gelfiltration column connected to an Äkta explorer (GE Healtcare), allowing change of buffer to 1 mM piperazin pH 9.5, 100 mM NaCl and separation of active monomeric 5'Nuc from aggregates and impurities.

Before use, the auxiliary enzymes were dialyzed overnight at 4°C against 50 mM HEPES buffer (pH 7.8) containing 1 mM DTE, with one change of buffer after 8 h.

Deposition of those polymers is achieved by local changes of the pH at the electrode surface caused by either electrolysis or change of buffer, resulting in discharged and insoluble Os-polymers that precipitate on the electrode surface.

The buffer was replaced with 5 ml secondary bacterial lysis buffer (0.25 mM EDTA, 1% N-lauroyl sarcosine and 1 mg/ml proteinase K) at 55°C for 48 hrs with a change of buffer after 24 hrs of incubation.

This implies that the activity of firefly luciferase is effectively quenched before measuring luminescence produced by the Renilla enzyme, an issue that is solved by a change of buffer composition and pH in the reaction tube.

The peaks containing the majority of the endo-xylanase activity were collected and further purified by ion exchange chromatography (using an ion exchange UNO Q1 column) with a linear gradient change of buffer stock A (20 mM TEA, pH 7.0) to buffer stock B (20 mM TEA, 1 M Nacl, pH 7.0).

The solution was dialyzed for 36 h against three changes of buffer (Tris-buffered saline, 30 mM benzamidine (Calbiochem), 0.1 mM PMSF (Pierce)) to remove the detergent, allowing liposomes to form.

After fixation, the samples were rinsed in three 15-min changes of buffer.

In order to improve the MAE, a trial of crystal deformation induced by changes of buffer layer compositions was taken.

The precipitate was redissolved in distilled water equal to half of the original volume and then dialyzed against 2 L PBS for 72 h at 4°C with two changes of buffer.

Blots were washed in 0.05% Tween-20 in TBS for 30 minutes at room temperature with three changes of buffer.