It has been speculated that a conformational change in the template-proximal anchor site is required for telomerase to transition into an elongation-competent complex [11].
Through this interaction, SmpB may orient the template sequence with respect to the TLD, causing conformational changes in the template, upstream sequence, and pseudoknot 1, positioning the resume codon in the A-site.
Lack of evidence for preplay could occur for two reasons, either: offline sequences of the target spatial representation were not generated, or the template shifted during learning (e.g., remapping) so that attempts to identify sequence matches are frustrated by changes in the template with experience.
We note that in the comparisons between this database and the original, and this database and itself (25 : 1 versus 25 : 1), that there is a distinct 2nd peak in the PMF close to a Hamming distance of 0; we attribute this to the comparison of images that are only slightly different (i.e., the compression does not result in much change in the iris template).
The two inclined TBs in the template on the left side leads to more pronounced shape change than the template in the middle, in which there is only one TB formed.
The fraction of Ade+ events among Ura+ transformants was significantly reduced in the pif1-m2 strain, but we did not observe a change in the ratio of template switching and chromosomal recombination associated with BIR, suggesting Pif1 plays no role in D-loop dissociation.
Changes in the silica template synthesis conditions did not result in substantial improvement of the strength of the microcapsules.
We then hypothesized that the stalling of the replication fork in the absence of Hdacs1,2 might be due to changes in the chromatin template, probably as a result of altered histone modifications.
