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Change in absorbance of the aqueous scale inhibitor solution.
One unit of POD activity was defined as the change in absorbance of 0.01 per minute at room temperature.
Graph shows the change in absorbance of the iodide band (351 nm) against the irradiation time for variously concentrated solutions of RB encapsulated in G2.5 PAMAM dendrimers.
The rate constant of the reduction process was determined by measuring the change in absorbance of the initially observed peak at 400 nm, for the nitrophelate ion as the function of time.
Figure 6 depicts the graphical representation of the change in absorbance of the iodide band (351 nm) against the irradiation time for variously concentrated solutions of RB encapsulated in G2.5 PAMAM dendrimers.
This latter can be further hydrolyzed by an exogenously added pyrophosphatase into two phosphate molecules that can be quantitatively measured with a standard colorimetric assay based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes.
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Among the different substrates, the changes in absorbance of the 5-nm nanoisland substrates were superior than that of the 1-nm substrates.
It was obvious that the maximum difference between changes in absorbance of the catalyzed and inhibited reaction was reached at 0.030 M.
Changes in absorbance of the reaction solution at 240 nm were determined after one minute.
The reduction of NAD to NADH was evaluated by detecting changes in absorbance of formazan salt at 490 nm.
These wavelengths (524, 589 nm) were chosen as these were isosbestic points not effected by changes in absorbance of myoglobin induced by oxygen desaturation [ 7].
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