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For further analysis, a fold change cut off 1.4 in mRNA expression was applied.
Genes with p-values less than 0.001 and an additional fold change cut off of ±2 were considered statistically significant.
Details about the experiments such as fold change cut off, Microarray platform, strains, etc. were obtained by carefully studying the journal articles.
However, if a 1.5 fold change cut off was used, a total of 503 genes were identified as HP regulated genes in the cerebral cortex.
From these normalised entity lists, a significance cut off of p ≤ 0.01 and fold change cut off of ≥ 2 were applied.
Listed are groups of gene-associated biological processes in bone marrow adipocytes compared with epididymal adipocytes (fold change cut off is 2.0).
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Probe sets were further filtered on the basis of a fold-change cut off of 2.0.
A functional analysis of DEG (P < 0.05, fold-change cut off ≥1.5 or ≤1/1.5 SCE vs. NUI) was undertaken with DIA using the KEGG pathway database.
Microarray analysis of adipose uncovered 144 DEG (P < 0.05, fold-change cut off ≥1.5 or ≤1/1.5 SCE vs. NUI) (82 down-regulated, 62 up-regulated) (Table 3).
Samples were displayed using an arbitrary 10-fold change cut-off for subsequent assessment for quantitative real time PCR analysis.
Approximately 260 probesets exhibited a fold change cut-off of 2.0 or more (Supporting Information Table S2).
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