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In orthopaedic surgery, a similar scenario is often observed in the case of suspected joint arthroplasty infections, and general guidelines have been implemented into clinical practice to improve the chances of isolating the responsible microorganisms [6]; however, in spinal surgery this approach is still far from being fully realized.
However, the antigen on the virus surface might be different from the purified protein because of the conformational changes, and this could decrease the chances of isolating phages that bind to the protein.
The latter approach should enhance the chances of isolating genuine mutagenic intermediates, a prerequisite for more definitive analysis of their functional and biochemical properties.
However this necessitates extensive screening, because the chances of isolating high-producing clones depend on the number of clones that have been screened.
To increase the chances of isolating TF genes from expressed sequences, especially for short or incomplete sequences and divergent members of gene families, we used less stringent criteria.
eak-7 (tm3188 ) and akt-1 (ok525 ) both harbor deletion mutations that are likely to be null alleles (Hertweck et al. 2004; Alam et al. 2010); these alleles were chosen to minimize the chances of isolating informational suppressors such as tRNA anticodon mutants or mutants defective in nonsense-mediated mRNA decay.
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After the 6th isolate was obtained in 2004, 5 additional monthly sampling sites were chosen at the mouths of major tributaries within the system to increase the chance of isolating more VDPVs and to more precisely locate the site of their entry into the sewage system (Secondary Sites #1-A to #1-E; Figure S1).
Because vaginal and rectal swabs are likely to yield diverse bacteria, selective broth is recommended to limit growth of other organisms, thus increasing the chance of isolating GBS (9 ).
Primers used for gene-specific screening for TPS (3CAR)- or P450 (CYP720B4 -containing CYP720B4 -containingssed in silico against other known memBACs of the large clonesr TPS-d family [ 15] and other members of the conifer-specific CYP720B family [ 20], respectively, to minimize the chance of isolating non-target members of these gene families.
The main divergence from such roles was seen in the work performed by specialist COs who occupied specific niches related to their areas of specialization, niches which provided greater autonomy and offered them the chance of isolating themselves from roles generally associated with the CO cadre.
Blood culture sampling in children uses smaller volumes (1 to 3 ml compared with 10 to 20 ml in adults), and although specially enriched culture media are used, the chance of isolating live organisms drops below 70% as compared with adults [ 74, 75].
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