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A homogenous cohort or case-control population and large number of data will increase the chance of validation of results in further studies.
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We observed only 2/26 (8%) false positives, suggesting that the other filter parameters (e.g., ≥ 2-fold difference in expression level) may have the increased the chances of validation by qRT-PCR.
The criteria were designed to maximize the chance of experimental validation but do not imply that all remaining TUs are artefactual.
Since the particular reverse transcriptase artifacts that may be produced by the SuperScript II enzyme will likely be different from those produced by the Roche Transcriptor enzyme, this reduces the chances of false validation due to such artifacts.
Considering robustness as an objective from the beginning of method development reduces significantly the chance of failure during the validation process.
Since there is no chance of performing a cross-validation analysis with motif matching, we choose self-consistency as a comparison strategy.
Although it is possible that lack of validation could occur by chance, based on these examples we believe that the TCGA validation set was adequately powered to detect effects of the size seen in previously published studies.
Although it is possible that lack of validation could occur by chance, this is highly unlikely for most markers in TCGA where the power of the validation analysis is expected to be >0.9.
It was this enormous act of validation.
"My years of validation save me".
It provided a key piece of validation.
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