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The chambers were placed on a shaker maintained at 37 °C and rotating at 110 rpm.
Courtship chambers were placed on a hotplate pre-warmed to 29°C, during which time the flies were introduced into each chamber.
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Experimental chambers were placed on the stage of an Axiovert 100 M inverted microscope attached to a LSM 510 laser-scanning unit (Zeiss, Oberkochen, Germany).
The experimental chambers were placed on the stage of an inverted microscope (Olympus IX81).
Vessel chambers were placed on the stage of an inverted Nikon Eclipse TS 100 (Nikon Instruments, Irvine, CA) microscope fitted with a video camera connected to a data acquisition computer.
Once the mice were ~25g (10 14 weeks old), the chambers were placed on the dorsal skinfold and the mice were segregated into separate cages and monitored daily.
The chamber was placed on a steel base frame driven 10 cm into each site one month prior to the start of the experiment.
Briefly, cells were pipetted in the inlet well, and the microfluidic growth chamber was placed on a Leica DMi8 inverted microscope equipped with a Leica DFC365 FX camera.
The filled chamber was placed on a stage preheated to 37°C.
The chamber is placed on a turntable and rotated at selected revolutions per minute.
The chamber was placed on a magnetic stirring plate and stirring intensity was used to manipulate flow speed.
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