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After incubation, the porous membrane was removed, and the migrated cells located in each bottom chamber were subsequently counted using a BIORAD T10 automated cell counter.
After 48h of incubation at 37°C, cells in the upper chamber were subsequently removed with cotton swabs and then stained with a solution containing 0.1% crystal violet and 4% formaldehyde.
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The debating chamber was subsequently closed, and MSPs moved to The Hub for one week, whilst inspections were carried out.
The chamber was subsequently incubated for 1 h at 37°C (5% CO2).
The sample chamber was subsequently evacuated under dynamic vacuum (approximately 10−4 mbar), and spectra were recorded at 25, 50, 100, 150, and 200 °C.
To distinguish between attached and non-attached PBMCs, the chamber was subsequently superfused with Hank's buffer containing 0.5%% bovine serum albumin.
After 2 min, the flow chamber was subsequently washed with TIRF buffer (See 'Materials and methods') to remove unattached filaments, free monomers, and GMPCPP.
It burned again in 1940, during a bombing raid, and a new roof and additional chambers were subsequently built.
Similar burial chambers were subsequently used by the Mycenaean civilization, suggesting that the founders of Mycenae may have come from Epirus and central Albania.
The lower chambers were subsequently exchanged in order to separate, to some degree, adhesive molecules and soluble factors in PSC-SN into two chamber systems.
After inoculation, all plants, including the mock-inoculated plants near 0 hpi, were incubated for 24 h in a 100% humid chamber, and were subsequently transferred to a growth chamber at 15°C with a 16-hour photoperiod.
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