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Simulations of an experiment of tissue differentiation in a mechanically loaded bone chamber were performed.
Dynamic adhesion assays in the flow chamber were performed as described previously [19].
Boyden chamber migration assays using a 10-well chamber were performed as described previously [ 25].
Chemotactic assays using the Boyden chamber were performed as described previously (Hernández-Miranda et al., 2011).
Intratumoural injections of EMCV or irradiated EMCV (300 J/cm for 90 s in a Spectrolinker chamber) were performed by injecting 5 µl of PBS-based virus suspension (2 × 10 virus particles/tumour).
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The calibration of pressure chamber was performed according to strictly defined steps.
In situ machining of the upper part of the chamber is performed on the machine tool in which the apparatus is located, in order to achieve high accuracy and geometric alignment of the system.
The Modified Boyden chamber was performed as described previously [23].
Photo documentation and cell counting by using a counting chamber was performed at every passage.
Photo documentation and cell counting by using a Neubauer counting chamber was performed at every passage.
To explore the effects of PO on bFGF-stimulated angiogenesis, the migration assay using the Boyden chamber was performed.
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