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In addition, mono-energetic correction factors for the low-energy free-air chamber were calculated.
The proportions of time spent by each mouse in the non-preferred chamber were calculated and used as the index for conditioned place preference.
Time spent in the previously non-preferred and preferred chambers were recorded and the percentages of time spent in the non-preferred chamber were calculated and compared within and between the groups.
On day 3 (testing day), 24 hours after conditioning, place preference was measured by returning the mice to the place conditioning environment for 5 min. The time spent (sec) in the air puff chamber and the safe chamber was measured and the proportions of time spent by each mouse in the air-puff chamber were calculated and used as the place preference index.
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The number of migrated cells in the lower chamber was calculated up to 72 h by microscope observation.
A histogram of the vertex distribution along the beam axis of the tracks crossing the chamber is calculated by the trigger system.
On the other hand, the acoustic and entropy sources coming from a combustion chamber are calculated from a three-dimensional Large Eddy Simulation (LES).
The total volume of the test chamber was calculated as 1278.2 cm3.
The relative humidity (%RH) inside the glass chamber was calculated as 82.93% by using the following relation [26]: % {text{RH}} = E_{text{w}} T1 )/E_{text{w}} T2 )times 100, (5 where E w (T1) (17.5 mm of Hg) and E w (T2) (21.1 mm of Hg) are the saturated water vapor pressure at the temperature of water (20 °C) and that of the composite film (23 °C), respectively.
The mean value from 5 fields per chamber was calculated from three independent experiments.
Cell recovery from the Matrigel layer was enhanced using the Cell Recovery Solution BD Biosciencess), and the percentage of cells in the lower compartment vs the upper chamber was calculated.
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