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All prepared activities were measured with a radionuclide calibrator CRC-15R (Capintec; manufactured in 1990) with an ionization chamber well of 6 cm diameter and 26 cm depth.
All of us who serve in this Chamber, well over 95% of us, have held other positions in government.
In brief, cell were plated in chamber well slides and cultured in Dulbecco's Minimum Essential Medium supplemented with 10% fetal bovine serum until confluent.
After 3 days of treatment, cultures were fixed in 4% paraformaldehyde, immunostained for MAP-2 (Sigma-Aldrich, St . Louis MO) and 20 digital images of each chamber well were captured at 400× magnification.
The animals tolerated the dorsal skinfold chamber well and showed no signs of discomfort.
Briefly, cells were plated in chamber well slides (2 × 10 cells per chamber) and allowed to adhere.
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Then CM-H2DCFDA (10 ìmol/L, Molecular Probes, USA) was added to chamber wells for 30 minutes.
The inserts were placed into the bottom chamber wells of a 24-well plate containing MEM with CCL15 as a chemoattractant.
NHK were seeded (6 × 10 cells/well) into the top chamber wells of the CIM-plate according to the manufacturer instructions.
Cold collagen solution (0.3 ml) was poured into 8-wells chamber wells (Nunc) avoiding bubble formation and warmed to 37°C to induce polymerization.
To detect the intracellular ROS production in cancer cells, 1 × 10 cells were seeded in chamber wells and cultured for 24 hours to reach >80% confluence.
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