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At 4% O2, the chamber was closed leaving a small dead space (0.5 cm) at the surface of the solution.
At 0% oxygen, the chamber was closed leaving a small dead space (0.5 cm) at the surface of the solution flushed with N2.
The experiment started as soon as the door to the sound attenuated chamber was closed, to rule out any influence of the experimenter.
The test lasts five minutes maximum; the door separating the departure chamber and the intermediate chamber was closed after the transition of the mouse (if a mouse did not enter after 2 minutes, it was gently guided toward the intermediate room).
The chamber was closed and incubated for 30 min at 37 °C.
Finally, the chamber was closed again with a new cover glass.
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After three minutes in which the chamber is closed for prayers, each day's session opens with the Speaker announcing departmental questions.
Seconds later, after the doors to the chamber are closed, a desperate, clamoring cry arises from within, then slowly subsides into moans and finally into silence.
The plasma device is mounted on a linear stepper motor which can adjust the distance accurately even when the chamber is closed.
Assuming a concentration C c (0) at the time t = 0 when the chamber is closed, the concentration in the chamber will change according to C c (t) = C s - [C s - C c (0)]e-αt.
When the chamber is closed, linear temperature and water vapour pressure gradients are established.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com