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In the earliest glimpse of the future while Fry is frozen in the cryonic chamber, time is seen passing outside the window until reaching the year 3000.
Fifteen minutes after sealing the chamber (time necessary to stabilize the sperm distribution and the attractant gradient), the sperm movement was recorded along the fields in the middle of the bridge.
Dead individuals were removed from their chamber; time of death, sex and standard length were recorded.
Nectar measurements both in the climate chamber and in the outdoor group were taken between 1230 and 1700 h on Day 3 of each temperature step, to give the plants in the climate chamber time to adjust to the changed conditions before sampling.
In these experiments, saturated cultures of SMR14350 were diluted 10,000-fold in M9 B1 0.1% glucose medium, grown to log phase shaking at 37°C, then GamGFP was induced by addition of 10 ng/ml doxycycline for 2 hr during shaking growth at 37°C prior to loading cells into the microfluidics chamber (time 0).
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For both groups, post hoc analyses revealed no difference in chamber times during the preconditioning baseline (p>0.05), but a significant difference in chamber times post conditioning (p<0.001).
Upon the addition of SDF-1 (50 nM) or RANTES (50 nM) to the cell chamber, time-lapse images were collected with multi-track mode (Zeiss 510), and CFP and Fluo-4 images were digitally separated.
The flux, J, was calculated as the product of the volume of the basolateral chamber times the rate of increase in basolateral FITC-BSA concentration determined from the linear region of [BSA]basolateral versus time plot.
Bills come to the floor from secret chambers, time for debate is limited, amendments are not allowed, and party-line up and down votes are taken.
He smashed his previous best of 10.23 to become the second fastest British junior of all time, overtaking former world junior champion Mark Lewis-Francis, with only Chambers' time of 10.06 set back in 1997 ahead of him.
Hale said he could not comment on Fadhel's statements about Chambers' time there.
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