Slices were transferred to a fixed-stage recording submerged chamber (standard ACSF flow rate of ~1 ml/min at 36 °C) in an upright microscope (Axioskop FS; Carl Zeiss, Germany).
Whereas these oscillations were reliably seen in interface slices, as previously reported (Fisahn et al., 1998; Kubota et al., 2003), no such activity persisted in submerged slices using standard flow rates (1.8 2.4 mL/min) either in the chamber standard or the modified submerged chamber (Figs 1A and B, and 2A).
In this paper, the authors investigate the suitability of compact semi-anechoic chambers (standard chambers, compliant for measurements at 3 m distance from the equipment under test) for reliable antenna factor calibrations.
Two paired standard and alternative window comparisons were as follows: (1) four-chamber: standard apical versus subcostal; and (2) three-chamber: standard apical versus parasternal long-axis.The global LPS intraobserver difference using the paired standard and alternative window comparisons was lower than the interangle difference in global LPS (-1.0 ± 0.1% vs -2.1 ± 2.4%).
Using naturally occurring cosmic rays, his scheme was to place spark chambers, standard equipment in the high-energy particle physics of this time, beneath the second pyramid of Chephren in a known chamber.
The plates were then placed in hypoxia chambers, standard cell culture incubators, or cultured in ambient air with or without the presence of the Hypoxia Inducible Factor 1-alpha (HIF-1α) stabilizer CoCl2 (100 μm, Sigma) for 4 h, removed and rinsed with PBS, followed by total protein collection in 4x sample buffer (20% glycerol, 4% SDS, 0.05% bromophenol blue, 160 mM Tris HCl, and 200 mM DTT).
The sampling rates were first evaluated in an exposure chamber under standard conditions.
She was criticised for not making a full statement about the report in the chamber – the standard procedure for a minister making an important announcement.
In the convection chamber, the standard k ε model and discrete ordinates (DO) radiation model were respectively used in the descriptions of turbulence characteristics and radiative heat transfer.
1.1 ml of this cell suspension were plated in triplicate into 35 mm Petri dishes (Greiner Bio-One, Frickenhausen, Germany) and incubated in a wet chamber at standard cell culture conditions.
By excluding consideration of the perpetrators' motives for killing the military-aged men, such as seeking to eliminate a military threat as the defense alleged, the Krstić chamber's standard for establishing specific intent to destroy the Bosnian Muslims, in whole or in part, was incomplete.
