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The cells were centrifuged, and resuspended in PBS, and added to a chamber slide where they were allowed to settle.
Once dissociated, cells were counted using a hemocytometer and plated at ~ 50,000 cells per well in an 8-well glass bottom chamber slide.
Images were acquired in this manner by sequentially visiting 4 wells of the chamber slide with a time of 6 minutes between imaging the same well.
Lab-TekTM chamber slide system was purchased from Thermo Scientific/Nalgene Nunc International (Rochester, NY, USA).
Following polymerization, embedded cultures were detached from the chamber slide and glued to Araldite blocks.
The chamber slide was incubated at 37 °C overnight for attachment and fresh media was added.
The uptake experiments were conducted after cell reaching confluence in a chamber slide.
Similar(4)
For immunofluorescence staining of live HEK cells chamber slides (4-well chamber-slide, Lab-Tek™, Nunc™) were used.
Chondrocytes were seeded at 5 × 104 cells per chamber in an 8-chamber slide.
MCF-7 and T47 D cells were seeded on a 4-chamber slide glass and incubated overnight.
Briefly, cells were seeded in a Lab-Tak eight-chamber slide (Thermo Scientific, Waltham, MA, USA) 1 day before experimentation.
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