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Briefly, the perfusion microbioreactor system consists of a microbioreactor chamber module, a plug module, and a bottom layer module).
In addition, in situ benthic oxygen uptake rates were determined with a microprofiler and a benthic chamber module.
The microbioreactor chamber module is composed of 9 cylindrical microbioreactor chambers with each microbioreactor having the same format as the well of a standard 96-well microplate (D: 7 mm; H: 7 mm).
TOU of the JTC colony center was determined with a small cylindrical benthic chamber module, which enclosed a sediment area of 284 cm (radius = 9.5 cm) together with 15 cm of overlying bottom water (equivalent to approximately 5 L).
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This level of swelling would pose technical challenges to the engineering of the target chamber modules; (3) Total tritium retention is predicted to be ∼0.5 5 kg in the Sombrero chamber within 0.67 FPY, which may call into question safety-status assumptions of the CFC-based chamber design.
Each chamber contains electronics that enable distributed sensing and mixing control allowing users to add new chamber modules with new capabilities with minimal hardware redesign.
Such a system comprises three parts – a waste heat recovery chamber, TEG modules and a cooling system.
It can be constructed without the need for a complicated bonding apparatus and tested without any module chamber.
A complete exposure system requires an aerosol generator, a dilution route and exposure chamber (also called module, plate or exposure device in certain set-ups) in which the biological culture is housed.
An in vitro aerosol exposure system supports such an approach, where a machine system will generate, dilute and deliver aerosols from cigarettes or e-cigarettes (or other nicotine delivery devices) to cell cultures at the air liquid interface (ALI) in a chamber or a module, mimicking a physiological aerosol exposure.
Briefly, MitoTracker® Red 580 (Molecular Probes, Invitrogen) stock solution (1 m m) was added into the culture medium in the module chamber to a final concentration of 100 n m.
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