Diffusion chamber kits (Millipore) were used for both in vitro and in vivo experiments, according to the manufacturer's instructions.
Invasion and migration of shControl and shLKB1 cells (2.5 × 10 cells per well) was measured using 24-well Matrigel invasion chamber kits (BD Biosciences, Bedford, MA, USA) according to the manufacturer's instructions.
Central morphologic reviews are performed on the formalin-fixed tissues sent via the dual-chamber kits which represent the mirror-image of samples of snap-frozen tumour and normal adjacent tissue also included in the kit.
Cooperative group affiliated institutions must send specimens to the BPC through air-express couriers within 24 h of procurement, using dual-chamber kits, which allow for simultaneous delivery of specimens at ambient and deep-frozen temperatures (Qualman et al, 2003).
Tonight's event was a candlelit dinner, with Mozart arias before every course, accompanied by a chamber orchestra, all kitted out in frock coats and powdered wigs.
Immunocytochemical analysis was performed on proliferating myoblasts and myotubes after 6 days of differentiation, grown on chamber slides using LSAB kit (DAKO, Glostrup, Denmark) according to manufacturer's instructions.
For invasion assays, 5 × 104 cells were plated in DMEM/1% FCS in a cell invasion chamber (Cell invasion assay kit, Chemicon International) in a 24-well plate, which contains an 8-μm pore size polycarbonate membrane covered with a thin layer of collagen matrix.
Invasion assays were performed in Matrigel-coated modified Boyden chambers (Cell Invasion Assay Kit, Chemicon/Millipore, Milan, Italy).
The terminal deoxynucleotidyl transferase (TdT) was used to incorporate fluorescein (FITC) labelled dUTP oligonucleotides to DNA strand breaks at the 3'-OH termini in a template dependent manner (TUNEL technique) for 90 min at 37°C in a humidity chamber using a commercially available kit (Roche Diagnostics, Penzberg, Germany).
