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Rats weighing 260 360 g were habituated to the fear conditioning chamber (context A) for 30 minutes one day prior to conditioning.
Rats in the behavior group were placed back into the fear conditioning chamber (context A) 24 hours later for analysis of freezing to the original training context (contextual fear memory).
Because mice displayed freezing behavior in response to fear, it is possible that mice do not show high freezing even if they remember the conditioning chamber (context test) or the tone (cued test).
Unit activities were acquired during the BL, EXT, and TEST sessions On Day 1, rats received 10 tone-alone (2 sec, 80 dB, 10kHz) presentations during the BL session after placement in the recording chamber (context A).
After 24 hours, rats were placed for 240 seconds in the training chamber (context test).
The environmental input consists in a discrete stimulus, i.e. a tone or the test chamber (context).
Similar(50)
At the start of each session, two mice (one WT, one 5-HTTOE) were brought to the testing room and each placed into one of two conditioning chambers (context 1 or context 2).
Individually, rats were first pre-exposed for 3 d to two experimental chambers (contexts) for 20 min/d.
A day later, the mice are tested separately for tone conditioning, in a different chamber (novel context) and exposed to the tone without receiving the shock.
The following day, the mice are tested for contextual conditioning by being reintroduced to the initial chamber (conditioned context) and being exposed to the tone, again without receiving the shock.
It is therefore possible that decreased anxiety-like behavior in CaMKIV KO mice shifted their coping behavior from a freezing response to a fleeing response and then resulted in lower non-specific freezing in the contextual test and during pre-tone and tone periods in the altered context chamber on Day 28.
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