Once sliced, the tissue was transferred to the testing chambers containing bath aCSF (32 °C), which flowed at 1 mL/min.
A three-dimensional tissue perfusion system (ADInstruments, Australia), including ML880 Recorder, four-channel bridge amplifier (ML224), four-chamber tissue bath (ML0146/10) and tension sensor (MLT01201) was used for the determinations of muscular tension and fatigue index.
The temperature was maintained at 37°C on a glass-bottomed bath chamber by a continuous, warmed perfusion and supplemental chamber heater.
Within the premises are a baradari (pillared pavilion) and a hammam (bath chamber).
After donning a yukata, the Japanese-style robe, in the dressing room, I entered the minimalist bath chamber, which contained two large rectangular tubs filled with the cedar enzyme fluff, smelling intensely -- but not unpleasantly -- like yeasty bread and hot cedar.
Recordings were performed in a perfused chamber, and the bath temperature was kept at 25°C by a temperature controller.
The coverslips were placed into a slotted bath chamber (37°C) at the microscope stage and cells were superfused with Tyrode alone for at least 10 min (control period).
Oocytes were placed in a bath chamber that was perfused with control ND96 bath solution containing (in mM), 96 NaCl, 3 KCl, 1 MgCl2, 2 Cand2, and 5 HEPES, titrated to pH 7.4 with NaOH.
For pH measurements, otocysts were transferred into a bath chamber, stabilized with a suction pipette and superfused with warm (37°C) bicarbonate-solution containing (mM): 135 NaCl, 25 NaHCO3−, 4 KCl, 1.5 CaCl2, 1 MgCl2 and 5 glucose, bubbled with 5% CO2 95% O2 to assume pH 7.3 7.4.
