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It is well established that FFPE tumour DNA samples are challenging to amplify and sequence due to damage resulting from the fixation process and due to sample heterogeneity.
Bisulfite conversion causes fragmentation of genomic DNA and it is technically challenging to amplify fragments greater than 500 bp in length [ 26].
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However, it was a challenge to amplify the 16S rRNA sequences of all the bacteria in environmental samples.
A major problem in tissue engineering is the availability of a sufficient number of cells with the appropriate phenotype for delivery to damaged or diseased cartilage and bone; the challenge is to amplify cell numbers and maintain the appropriate phenotype for tissue repair and restoration of function.
In addition a neonatal LPS challenge was reported to amplify the immune response to experimental TNBS-induced colitis in rats [43].
That's what Twitter should recognize as its greatest challenge: the use of the service to amplify its shortcomings.
The assessment of the quality of proteins in tissues, particularly very small samples, is a significant challenge since there is no current method to amplify proteins for analysis.
Our task over the coming decades is to amplify their opportunities while mitigating their challenges.
So my challenge is to get us back to those roots, to amplify those roots and to let the organization run with it".
To amplify the training effects of a bridging exercise and specifically challenge stability mechanisms, labile surfaces such as gymnastic balls used to be advised [ 22, 23].
However, high sequence variability of the viral agents posts a challenge to the design of robust universal virus-specific primer sets to amplify various virus variants.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com