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The overnight cultures were diluted to an OD600 of 0.02 in LB medium containing 50 µg/ml ampicillin and challenging substances of different concentrations, and then grown at 37°C.
Different concentrations of challenging substances were tested and concentrations that allowed growth of the liquid library were then used for selection.
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It is rather that he seeks to fill them with meaty, challenging substance.
There was a relatively close match between physiologic and symptomatic responses during exposure to challenge substances.
We considered only the tonic responses while assessing a positive response to challenge substances in our pilot study.
Of the physiologic measures, only skin conductance was a consistent indicator of adaptation and response to challenge substances postadaptation.
Figure 5 presents the percentage of individuals with ES having a specific symptomatic response to each of three challenge substances: glue, body wash solution, and dryer sheet.
Otherwise, we might obtain erratic physiologic or symptomatic responses because of testing conditions, which would make it impossible to differentiate challenge substances from placebos.
We disregarded the symptoms that occurred consistently during the adaptation sessions (baseline symptomatic responses) while computing a response toward challenge substances.
His Democratic supporters have also refrained from challenging the substance of the allegations against Mr. Torricelli, and have instead aimed their criticism at Mr. Chang's credibility and the press coverage of the investigation.
As support, rather than challenging the substance of Christensen's case, Lepore takes a superficial, snarky stab at some of his examples and quickly migrates to another topic.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com