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Particular safety concerns included crash history, especially the high percentage of motorcycle crashes, the increase in traffic volumes as well as high operating speeds, especially at some locations with challenging alignment.
This tremendously simplifies computationally challenging alignment problems in species with high levels of genetic diversity.
By choosing appropriate REs, repetitive regions of genomes can be avoided and lower copy regions can be targeted with two to three fold higher efficiency [12], [19], which tremendously simplifies computationally challenging alignment problems in species with high levels of genetic diversity.
Challenging alignment optimization problems were selected from the literature according to the following criteria: the global sequence identity between the target and its template must be lower than 35%, a crystal structure must be available for the target, and the sequence based and structural alignments must differ in one or more regions encompassing secondary structure elements (SSEs).
This sequence set consists of four DNA-binding proteins and is a challenging alignment example as there is only weak similarity at the primary sequence level.
These attributes of substrate and product permit processing and imaging of a small number of relatively thick sections (200 400 um thickness), thus minimizing the technically challenging alignment of neuronal processes between sections.
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Non-conserved RAA regions of rDNA are especially challenging for alignment because both secondary rDNA structures and sequences evolve in these regions.
The fifteen predictors were assessed on ten challenging local alignments optimization problems of both α helix and β sheets, among which challenging CASP cases with no sequence identity between the template and the target.
We are grateful for Robert Sinclair's insights regarding challenging sequence alignments.
Because availability of known microbial genome references are limited and de novo assembly of metagenome sequences is challenging, the alignment-free sequence signature method is a promising approach for analyzing multiple metagenomic samples.
This problem even challenged the alignment of some ORFs from the closely related P. caudatum and P. tetraurelia.
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